VAHTS® RNA Multi-PCR Library Prep Kit with SARS-CoV-2 Panel

NA211

Two rounds of PCR amplification are
adopted to complete target amplification and enrichment. It only takes 5 h from viral genomic sample acquisition to obtaining complete
sequencing library, and the manual operation time is less than 1.5 hours. This product is compatible with the starting template
range of 16 Ct to 38 Ct, and the constructed library is suitable for sequencing analysis on the Illumina platform.
The customized panel in this kit has a coverage of 99.7% of the whole genome. With multi-PCR amplification reagents, the uniformity
can reach 90%, which can effectively detect the mutation and deletion sites. The optimized combination of the reagents enables easy,
stable and reliable RNA library construction. All reagents provided in the kit have undergone strict quality control and functional
verification, ensuring the optimal stability and repeatability of library construction.

Components

Components NA211-01 NA211-02
(24 rxns) (96 rxns)
 10× RT Mix 48μl 192 μl
 HiScriptIII Enzyme Mix 48μl 192 μl
 RandomHexamers 24μl 96μl
 RNase-freeddH2O 1 ml 1 ml
 4 × VAHTSMulti-PCR Mix 120μl 480 μl
 Primer Pool1 24μl 48μl
 Primer Pool2 24μl 48μl
 VAHTSHiFiAmplificationMix 960μl 4 × 960 μl
 TE 1 ml 4 × 1 ml
▲Refer to Appendix for the second round PCR primers.

Panel (V3.0) information:

Number of pairs of primers: 247

Pool 1 primer pairs: 125

Pool 2 primer pairs: 122

Net product size range: 54-232 bp

Product size range (including library sequence): 191-369 bp

Cross-compatible with known variants of concerns

Genomic coverage: >99.7%