Collection: Site-directed Mutagenesis

Mutagenesis refers to the process of inducing changes in the DNA sequence of an organism. Our mutagenesis kits provide the necessary tools and reagents to introduce specific mutations into a DNA sequence of interest. The three key methods to introduce nucleotide changes into a target gene in an efficient and targeted way include:

1. Site-Directed Mutagenesis:

Designed for the introduction of specific, predetermined mutations into a DNA sequence; these kits often use synthetic oligonucleotides containing the desired mutations and DNA polymerases with proofreading capabilities to incorporate the changes accurately.

2. Random Mutagenesis:

Random mutagenesis kits introduce random nucleotide changes across a target gene typically using error-prone DNA polymerases, such as mutagenic polymerases, to introduce random mutations during DNA amplification.

3. Saturation Mutagenesis:

Saturation mutagenesis aims to generate all possible nucleotide changes at a specific position within a gene. Our kits use a combination of synthetic oligonucleotides, each containing a different nucleotide at the target position, to create a library of mutants.

Our mutagenesis kits enable researchers to generate genetic variants, explore gene function, study drug interactions, explore structure-function relationship of genes, model diseases, direct evolution, perform gene knockout studies, prepare NGS libraries, and engineer proteins for various applications.