TransScript® II Green One-Step qRT-PCR SuperMix

AQ311-01

20% Off NOW until March 31st as part of our Fiscal Year End Sale 2025! Buy this reverse transcription product, get 10% off towards PCR plates or 8-strip tubes, or sealing film.

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TransScript^® II Green One-Step qRT-PCR SuperMix AQ311 is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers to achieve one step from reverse transcription to qPCR in a single tube.

Highlights

• TransScript^® II Green One-Step RT/RI Enzyme Mix is used to efficiently synthesize first-strand cDNA from RNA and 2×PerfectStart^TM Green One-Step qPCR SuperMix is used for qPCR, enabling simple procedures to minimize the chance of contamination.
• High sensitivity and high specificity to ensure accurate results.

Applications
• High-copy and low-copy gene detection
• Detection of RNA virus or trace amounts of RNA

Storage
at -20°C away from light

Shipping
Ice/Gel Packs (~2-8℃)

Product Contents

	100rxn x 20ul	400rxn x 20ul	1000rxn x 20ul
Component	AQ311-01	AQ311-02	AQ311-03
TransScript® II Green One-Step RT/ RI Enzyme Mix	40 μl	160 μl	400 μl
PerfectStart® Green One-Step qPCR SuperMix (2×)	1 ml	4×1 ml	10×1 ml
Passive Reference Dye (50×)	40 μl	160 μl	400 μl
RNase-free Water	1 ml	4×1 ml	10×1 ml

Citations

Synaptotagmin-11 inhibits cytokine secretion and phagocytosis in microglia[J]. Glia, 2017, 65(10):1656-1667. 

A differential sequencing-based analysis of the C. elegans noncoding transcriptome[J]. RNA, 2012, 18(4):626-639.

 Regulatory elements of Caenorhabditis elegans ribosomal protein genes[J]. BMC Genomics, 2012, 28(13):433.4.The 

loss of MiR-139-5p promotes colitis-associated tumorigenesis by mediating PI3K/AKT/Wnt signaling[J]. The International Journal of Biochemistry & Cell Biology, 2015, 69:153-161.

 Identification of Intermediate-Size Non-Coding RNAs Involved in the UV-Induced DNA Damage Response in C. elegans[J]. PLoS ONE, 2012, 7(11):e48066.

Customer Reviews

One step qRT-PCR

Our lab compared between two different companies and we found a similar results and i would recommend this product as its cheaper.