SERVICEBIO Tissue Homogenizer Low Temperature

SWE-FP

Product Introduction
SWE-FP is a newly upgraded low-temperature homogenizer, which can reach a temperature as low as -50℃ in the chamber and also equipped with a freezing table. Adopt high-speed reciprocating motion of the vertical shaking system, the frozen sample in the grinding tube collides with the grinding bead, and the resulting grinding shear force and impact force completely break down the tissue. Up to 192 samples can be processed simultaneously in one minute, making it a dedicated device that meets the requirements of rapid processing of multi-sample.

Product Features

1. Slider drive, no wear shaft, noise stability and durability;
2. Equipped with low temperature freezing table. It can meet the needs of sample storage, electrophoresis, membrane transfer and other low-temperature experiments
3. Imported compressor, lowest cooling temperature of -50℃,low temperature grinding throughout the process, reducing degradation of proteins and RNA.
4. Up to 192 samples can be processed simultaneously in 1 minute (24 samples can be processed with standard),efficiently and effectively completing sample grinding with high throughput.
5. Multiple adapters to choose.

Technical Parameters

1. Dimensions: Length 650mm,Width 420mm,Height 430mm
2. Weight:54 kg
3. Screen size:5 inches
4. Temperature:-50℃
5. Temperature control precision:±1℃
6. Working time:Runtime0-9999s,pausetime0-999s,andruntimes1-99times

Parameters Comparison

Cat. No.	KZ-III	KZ-III-F/FP	KZ-5F-3D	SWE-FP
Operating mode	The vertical shaking system moves back and forth at high speed.	the vertical shaking system moves back and forth at high speed	Move in a "∞" shaped three-dimensional motion in space.	The vertical shaking system moves back and forth at high speed.
Temperature	Room temperature	-10℃/-40℃	-40℃	-50℃
Temperature control precision	N/A	±1℃	±1℃	±1℃
Standard spec.	24*2 mL	24*2 mL	24*2 mL	24*2 mL
Maximum sample volume	60 pcs	60 pcs	24 pcs	192 pcs
Adapter specification	24x0.5mL. 24x2mL. 32x2mL. 48x2mL. 60x2mL. 12x5mL. 10x10mL. 6x5mL (steel can). 4x30mL (steel can). 2x50mL (steel can) and 8*15mL (steel can)		24*2mL. 12*5mL(Optional )	24x0.5mL.24x2mL.48x2mL. 32x2mL.60x2mL.96x2mL. 12x5mL.10x10mL.4x30mL(steel can). 6x5mL(steel can).8x15mL(steel can) 2x50mL(steel can).96x2mL(2pcs 96-deep well plate)
Freezing table	N/A	N/A	N/A	YES
Adapter fixing method	Nut	Nut	Knob cover with safety locking device	Nut
Working time	0-99min99s	0-99min99s	0-99min99s	0-99min99s
Dimensions	365*265*396mm	370*365*500mm		650*420*430mm
Screen size	5 inches	5 inches	5 inches	5 inches
Emergency stop button	YES	YES	YES	YES
Electromagnetic safety lock	Screen click control with automatic power-off unlocking. the cover cannot be opened during operation until the grinding program ends, fully protected.
Maximum feeding size	No requirements, adjustable according to the adapter.
Weight	30.7kg	45kg	52kg	54kg
Power parameters	200-240VAC,50-60 Hz, 200 W	200-240VAC,50-60 Hz, 400 W	200-240V AC,50-60 Hz, 400 W	200-240VAC,50-60 Hz,500 W
Cooling method	Low temperature pre-cooling: adapter can reach -80℃ or liquid nitrogen pre-cooling	Compressor cooling	Compressor cooling	Compressor cooling
Transport screws	Yes (2 at the bottom)	Yes (2 at the bottom)	N/A	Yes (2 at the bottom)
Advantages	Small size, cost-effective	Compatible with various adapter specifications. Freezing function, lowest temperature can reach -40℃.	Higher grinding efficiency, reducing tissue degradation. Freezing function, lowest temperature can reach -40℃.	Lower cooling temperature can reach -50℃. low temperature freezing table, Protects samples and meets low-temperature needs for Western Blot experiments, electrophoresis, and transfer membranes.

Unboxing and Installation

Remove the high-speed low-temperature tissue grinder from the packaging box and place it in a suitable location. Confirm that all components are complete.
Use the wrench provided in the accessories to remove the two fastening screws at the bottom of the instrument. (Before the first grinding operation, make sure to remove the fastening screws.)


Place the machine on a level and flat laboratory bench to prevent vibrations during operation.
Let it stand for 10 hours to ensure the coolant is in place. Connect the power supply (200-240V) and turn on the power switch at the back of the grinder.
Turn on the compressor cooling switch and wait for 10-15 minutes. Observe if the temperature in the grinding chamber reaches the set temperature.
6.If the temperature reaches the set temperature, install the adapter and nut correctly, then cover the top and start the machine.

Sample Preparation
The adapter can be used to grind 96 samples simultaneously in a 96-well plate. The adapter includes a base that can hold a 96-well plate, a top cover, and a locking nut.
Take the samples to be ground and place them in the grinding tubes. It is recommended to use no more than 100mg of sample. Add the corresponding extraction solution, ensuring that the total volume of the sample and extraction solution is within half of the overall volume. Add a few appropriately sized grinding beads. To ensure grinding efficiency and quality, for larger volume samples, it is recommended to cut them into smaller pieces using scissors.
Unscrew the locking nut and remove the top cover. Place the grinding tubes into the adapter, then attach the top cover and tighten the locking nut in a clockwise direction. (The placement of the tubes should follow the principles of symmetry and balance.)

Operation Steps
Plug the power cord of the grinder into a grounded 220V power outlet, and turn on the power switch at the back of the grinder.
After the startup interface is displayed, wait for about 5 seconds to enter the main operating interface.
Once the grinder is turned on, the cooling system starts running. Place the grinding tubes containing the samples, tighten the adapter, and cover the top of the grinder.
Set the operating frequency, time, and number of cycles. Click the "Temperature Selection" button to choose the temperature mode and set the desired temperature for the grinding chamber or the cooling platform.
Click the start button to begin the grinding process. The instrument will automatically stop once the timer reaches zero.

Equipment Maintenance
Perform the following routine maintenance after each use to ensure the reliable operation of the grinder.
Make sure to turn off the power and unplug the power cord before cleaning.
If solvents, salt water, acidic or alkaline solutions are spilled on the grinder, immediately wipe them clean with a damp cloth to avoid damaging the instrument.
Do not use high-pressure sterilization on any part of the grinder, including the adapter.
The components of the adapter should be cleaned after use. Use an appropriate cleaning agent, rinse with distilled water, and then wipe dry with a paper towel.
After using an appropriate cleaning agent, wipe the grinder clean with a soft cloth.
The following disinfectants and cleaning agents are recommended for cleaning the grinder and adapter:
a) Ordinary cleaning agent
b) Neutral detergent
c) 70% alcohol content 

 Equipment List

No.	Product Name	Model/Specification	Quantity	Remarks
1	Tissue Homogenizer	SWE-FP	1
2	Standard Hollow Adapter	2mL*24	1	Can process 24 samples at once
3	Beads Pen	SYM-04Z	1	Suitable for 2mL grinding tubes, zirconia beads 4mm
4	Beads Pen	SYM-04B	1	Suitable for 2mL grinding tubes, steel beads 4mm
5	Beads Pen	SYM-03B	1	Suitable for 2mL grinding tubes, steel beads 3mm
6	2mL Centrifuge Tube		1	Tube wall is thickened, material strength is higher, and there is no RNA enzyme.

Protein Extraction

Product Name	Grinding Beads	Frequency	Grinding Time	Pause Time	Cycle times	Recommended Extraction Solution
Plant Stems, Leaves	Stainless Steel Beads 3mm*3, 4mm*1	60Hz	60s	15s	3	Protein Extraction Solution
Heart, Liver, Spleen, Lungs, Kidneys, Brain of Mice and Rats	Stainless Steel Beads 3mm*2, 4mm*1	60Hz	30s	15s	2	Protein Extraction Solution
Stomach, Intestines, Muscles, Fat, Pancreas of Mice and Rats	Stainless Steel Beads 3mm*2, 4mm*1	60Hz	45s	15s	2	Protein Extraction Solution
Skin, Ears, Tail, Blood Vessels of Mice and Rats	Stainless Steel Beads 3mm*3, 4mm*2	70Hz	60s	20s	3	Protein Extraction Solution
Cartilage, Hair, Hard Bones of Mice and Rats	Stainless Steel Beads 3mm*4, 4mm*2	70Hz	90s	20s	3	Protein Extraction Solution

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

 RNA Extraction

Product Name	Grinding Beads	Frequency	Grinding Time	Pause Time	Cycle times	Recommended Extraction Solution
Plant Stems, Leaves	Zirconia Beads 3mm*3, 4mm*1	60Hz	60s	15s	3	RNA Extraction Solution
Heart, Liver, Spleen, Lungs, Kidneys, Brain of Mice and Rats	Zirconia Beads Beads 3mm*2, 4mm*1	60Hz	30s	15s	2	RNA Extraction Solution
Stomach, Intestines, Muscles, Fat, Pancreas of Mice and Rats	Zirconia Beads Beads 3mm*3, 4mm*2	60Hz	45s	15s	2	RNA Extraction Solution
Skin, Ears, Tail, Blood Vessels of Mice and Rats	Zirconia Beads Beads 3mm*4, 4mm*2	70Hz	60s	20s	3	RNA Extraction Solution
Cartilage, Hair, Hard Bones of Mice and Rats	Zirconia Beads Beads 3mm*3, 4mm*4	70Hz	90s	20s	3	RNA Extraction Solution

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

For RNA extraction, the grinding time is extended compared with that of protein extraction, and RNA extraction solution is added to the grinding, the specific grinding time depends on the amount and type of tissue to be ground. Samples difficult to break can be repeated grinding 2-3 times, the specific situation can be adjusted appropriately according to the experiment.
Strong and Tough Tissue Grinding
For samples that are difficult to grind (e.g. cartilage, hair, etc.), we recommend using a 2mL grinding tube. Cut the sample into 1-2mm pieces, add the appropriate number of grinding beads, cover the tube tightly and freeze in liquid nitrogen for 2-3min without extracting solution, dry grind for 60 seconds at 70Hz, then add extracting solution to the tube and repeat the grinding once. If the grinding result is not satisfactory, the tube can be frozen again and the above experimental steps can be repeated.
Bone Tissue Grinding
For bone tissue, use the special steel grinding jar and adapter. The bone tissue is first frozen in liquid nitrogen for 5-10min, then put into the grinding jar, add the steel beads, and grind for 180 seconds at 70Hz (set cycle grinding). After grinding, take out the finished tissue, put it into a 2mL grinding tube, add the extraction solution, two 3mm grinding beads and homogenize for 60 seconds.      

      

Cat. No.	Product Name	Spec.

KZ-5F-3D	3D Tissue Homogenizer (-40℃)	(-40℃)
KZ-III	Tissue Homogenizer (Room Temperature)	Room Temperature
KZ-III-96	Tissue Homogenizer Room Temperature (96-well Plate)	Tissue Homogenizer Room Temperature (96-well Plate)
KZ-III-F	Tissue Homogenizer Low-temperature (-10℃)	(-10℃)
KZ-III-FP	Tissue Homogenizer Low-temperature (-40℃)	(-40℃)
KZ-III-YLS	Water-quality Chlorophyll Homogenizer	Room Temperature
SWE-FP	Tissue Homogenizer Low Temperature (-50℃, Freezing Table)	(-50℃, Freezing Table)