T7 RNAi Transcription Kit

TR102-01

The purified transcript can be used for
RNAi experiments mediated by cationic liposome, calcium phosphate coprecipitation, electroporation, DEAE-dextran and microinjection.

Kit Composition

Components	TR102-01 50 rxn	TR102-02 100 rxn
T7 Enzyme Mix	50 μl	100 μl
10 × Transcription Buffer	50 μl	100 μl
10 × Annealing Buffer	250 μl	500 μl
NTP Mix	200 μl	400 μl
DNase I	25 μl	50 μl
RNase T1 (100 U/μl)	25 μl	50 μl
RNase T1 Dilution Buffer	300 μl	600 μl
Control Template*	5 μl	10 μl
RNase-free H2O RNA Clean Beads	5 ml 2 ml	5 ml 4 ml

Storage: Store all the components at -20℃.