Size:20ug. Other sizes are also available. For further information, please contact us.
Research Areas:Others
Uniprot ID:O18425
Gene Names:N/A
Organism:Eisenia fetida (Red wiggler worm)
AA Sequence:MSSRAGIAEGYEQIEVDVVAVWKEGYVYENRGSTSVEQKIKITKGMRNLNSETKTLTASHSIGSTISTGDLFEIATVDVSYSYSHEESQVSMTETEVYESKEIEHTITIPPTSKFTRWQLNADVGGADIEYMYLIDEVTPIGGTLSIPQVIKSRAKILVGREIYLGETEIRIKHADRKEYMTVVSRKSWPAATLGHSKLYKFVLYEDMYGFRIKTLNTMYSGYEYAYSSDQGGIYFDQGSDNPKQRWAINKSLPLRHGDVVTFMNKYFTRSGLCYYDGPATDVYCLDKREDKWILEVVKP
Expression Region:1-300aa
Sequence Info:Full Length
Source:E.coli
Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged
MW:41.6 kDa
Alternative Name(s):Fetidin (Hemolysin) (Lysenin-3) (efL3) (LRP-2)
Relevance:Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells. Has hemolytic activity. It also has antibacterial activities against B.megaterium.
Reference:"Relationship between hemolytic molecules in Eisenia fetida earthworms." Prochazkova P., Silerova M., Felsberg J., Joskova R., Beschin A., De Baetselier P., Bilej M. Dev. Comp. Immunol. 30:381-392(2006)
Purity:Greater than 85% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:Pore-forming toxin that specifically binds sphingomyelin in the plasma membrane of various cells (By similarity). Has hemolytic activity. It also has antibacterial activities against B.megaterium.
Involvement in disease:
Subcellular Location:Secreted, Target cell membrane
Protein Families:Lysenin family
Tissue Specificity:Expressed by coelomocytes.
Paythway:
HGNC Database Link:
UniGene Database Link:
KEGG Database Link:
STRING Database Link:
OMIM Database Link:
Lead Time Guidance:3-7 business days