Size:100ug. Other sizes are also available. For further information, please contact us.
Research Areas:Others
Uniprot ID:P07486
Gene Names:Gapdh1
Organism:Drosophila melanogaster (Fruit fly)
AA Sequence:MSKIGINGFGRIGRLVLRAAIDKGASVVAVNDPFIDVNYMVYLFKFDSTHGRFKGTVAAEGGFLVVNGQKITVFSERDPANINWASAGAEYVVESTGVFTTIDKASTHLKGGAKKVIISAPSADAPMFVCGVNLDAYSPDMKVVSNASCTTNCLAPLAKVINDNFEIVEGLMTTVHATTATQKTVDGPSGKLWRDGRGAAQNIIPAATGAAKAVGKVIPALNGKLTGMAFRVPTPNVSVVDLTVRLGKGATYDEIKAKVEEASKGPLKGILGYTDEEVVSTDFFSDTHSSVFDAKAGISLNDKFVKLISWYDNEFGYSNRVIDLIKYMQSKD
Expression Region:1-332aa
Sequence Info:Full Length
Source:E.coli
Tag Info:N-terminal 6xHis-GST-tagged
MW:66.9 kDa
Alternative Name(s):Glyceraldehyde-3-phosphate dehydrogenase I (GAPDH I) (Gadph-1)
Relevance:
Reference:"Promoter-associated pausing in promoter architecture and postinitiation transcriptional regulation." Lis J. Cold Spring Harb. Symp. Quant. Biol. 63:347-356(1998)
Purity:Greater than 85% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:
Involvement in disease:
Subcellular Location:Cytoplasm
Protein Families:Glyceraldehyde-3-phosphate dehydrogenase family
Tissue Specificity:
Paythway:
HGNC Database Link:
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Dm&CID=23224
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?dme:Dmel_CG12055
STRING Database Link:https://string-db.org/network/7227.FBpp0087977
OMIM Database Link:
Lead Time Guidance:13-23 business days