Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:RPS6KA2,RPS6KA3,RPS6KA1,RPS6KA6
GeneID:6196,6197,6195,27330
Uniprot:Q15418/P51812/Q15349/Q9UK32
Specificity:Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/S218/232.
Immunogen:A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/S218/232.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Mouse,Rat
Prediction:Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
Application:WB,IF/ICC,ELISA(peptide)
Dilution:WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Conjugate:Unconjugated
RRID:AB_2841947
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:DF8743-BP
Des# Blocking Peptide:Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Blocking peptide
Blocking Peptide Price:$549 CAD