|
Description |
pEASY ®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion. •5 minutes fast ligation of Taq-amplified PCR products. •Ampicillin resistance genes for selection. •Easy blue/white selection. •T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. •T7 promoter and SP6 promoter for in vitro transcription. •Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. |
|
Storage |
At -20°C for nine months |
|
Kit Content |
|
Component |
CT301-01 |
CT301-02 |
|
(20 rxns) |
(60 rxns) |
|
|
pEASY ®-T3 Cloning Vector (10 ng/μl) |
20µl |
3×20µl |
|
Control Template (5 ng/μl) |
5µl |
5µl |
|
Control Primers (10 μM) |
5µl |
5µl |
|
M13 Forward Primer (10 μM) |
50µl |
150µl |
|
M13 Reverse Primer (10 μM) |
50µl |
150µl |
|
SR Primer (10 μM) |
||
Note: this kit does not include competent cells.
References:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching. | Nature Biotechnology | 39.08 | 2013 Sep | The original link | |
| Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice | Genome Biology | 10.465 | Yu-Chan Zhang, et al. | 2014 Dec | The original link |
| Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years | journal of hazardous materials | 6.065 | Shuang Peng,et al. | 2017Qct | The original link |
| Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli | Scientific Reports | 5.578 | Jie Gao, et al. | 2016 Jan | The original link |
| Identification of ethylene-responsive genes in ethrel-treated shoot apices of cucumber by suppression subtractive hybridization | Plant Molecular Biology Reporter | 5.319 | Jin X, et al. Department of Horticulture, Northeas | 2011 Dec | The original link |