pEASY®-T1 Simple Cloning Kit

CT111

Description pEASY ®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 minutes fast ligation of Taq-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •SR primer and M13 forward primer for sequencing. •T7 promoter for in vitro transcription. Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage At -20°C for 9 months Kit Content Component CT111-01 CT111-02 (20 rxns) (60 rxns) pEASY ®-T1 Simple Cloning Vector (10 ng/μl) 20 µl 3×20 µl Control Template (5 ng/μl) 5 µl 5 µl Control Primers (10 μM) 5 µl 5 µl M13Forward Primer (10 μM) 50 µl 150 µl M13Reverse Primer (10 μM) 50 µl 150 µl SR Primer (10 μM) 50 µl 150 µl 10×100 μl 30×100 μl

Please note: this kit does not include competent cells. 

References:

Literature	Journal	IF	Author	Date	Link
A transgenic strategy for controlling plant bugs(Adelphocoris suturalis) through expression of double-stranded RNA homologous to fatty acyl-coenzyme A reductase in cotton	new phytologist	7.21	Jing Luo,et al.	2017Jun	The original link
A transposon-directed epigenetic change in ZmCCT underlies quantitative resistance to Gibberella stalk rot in maize	new phytologist	7.2	Chao Wang et al National Maize Improvement Centre	2017 Jul	The original link
Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification	analytical chemistry	6.32	Yi Hu,et al.	2016Nov	The original link
Nitrogen deposition mediates the effects and importance of chance in changing biodiversity	Molecular Ecology	6.275	Ximei Zhang, et al. State Key Laboratory of Vegeta	2011 Jan	The original link
Characterization of Cyclooxygenase-2 and its induction pathways in response to high lipid diet-induced inflammation in Larmichthys crocea	Scientific Reports	5.578	Tianjiao Wang, et al.	2016 Feb	The original link