pEASY®-Blunt M3 Expression Kit

CM311-GB

Features:

• 5 minutes fast ligation of Pfu-amplified PCR products. • Enhanced CMV promoter for higher protein yield. • CMV forward primer and TK polyA reverse primer for sequencing. • C-terminal Flag-tag for protein detection. • Neomycin resistance gene for stable cell line selection. Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. • M3 Expression Plasmid included as negative control.
Storage	Competent Cell at -70°C for six months; others at -20°C for nine months
Kit Content

Component	CM311-01 (10 rxns)
pEASY ®-Blunt M3 Expression Vector (15 ng/μl)	10 µl
M3 Expression Plasmid (Negative Control) (15 ng/μl)	10 µl
MControl Template (5 ng/μl)	10 µl
MControl Forward Primers (10 μM)	10 µl
MControl Reverse Primers (10 μM)	10 µl
CMV Forward Primer (10 μM)	50 µl
TK PolyA Reverse Primer (10 μM)	50 µl
Trans1-T1 Phage Resistant	5×100 μl
Chemically Competent Cell