pEASY®-Blunt Cloning Kit

CB101-GB

Description	pEASY ®-Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •T7 promoter, M13 forward and M13 reverse primers for sequencing. •T7 promoter for in vitro transcription. Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage	Competent Cell at -70°C for six months; others at -20°C for 9 months
Kit Content

Component	CB101-01	CB101-02
	(20 rxns)	(60 rxns)
pEASY ®- Blunt Cloning Vector (10 ng/μl)	20µl	3×20 µl
Control Template (5 ng/μl)	5µl	5µl
Control Primers (10 μM)	5µl	5µl
M13 Forward Primer (10 μM)	50µl	150µl
M13 Reverse Primer (10 μM)	50µl	150µl

Please note: this kit does not include competent cells. 

References

Literature	Journal	IF	Author	Date	Link
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew	Nature Biotechnology	39.08	Yanpeng Wang, et al.	2014 Jul	The original link
D14?CSCFD3-dependent degradation of D53 regulates strigolactone signaling	Nature	38.597	Feng Zhou,et al.	2013 Dec	The original link
??Perfect?? designer chromosome V and behavior of a ring derivative	Science	34.661	Xie Z, et al. Key Laboratory of Systems Bioenginee	2017 Mar	The original link
Targeted genome modification of crop plants using a CRISPR-Cas system	Nature Biotechnology	32.44	Qiwei Shan,et al.	2013 Aug	The original link
Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds	Cell Research	12.413	Junqing Ye, et al.	2015 Dec	The original link