20 µL per reactions for 10 mL reactions for the 500 rxn size, and 20 mL reactions for the 1000 rxn size.
Using the Lyse n Amp(TM) Mouse Genotyping Kit, there is no need of cumbersome homogenization, crushing, overnight digestion, phenol chloroform extraction, DNA precipitation or column purification operations, which greatly shortens the experimental time. The extracted genomic DNA can be used directly as template for PCR amplification to evaluate if the mouse contained the target DNA. This Kit supplies both the lysis reagents and 2 × Taq Plus Master Mix (Dye Plus) (P212), which includes a high-performance Taq Plus DNA Polymerase, dNTP, and optimized buffer system to ensures high amplification efficiency of target DNA. Because Taq DNA Polymerase has the 3' terminal transferase activity, the dA overhang defines each of the 3' ends of the PCR product. Therefore, the resulting PCR product can be cloned into any T vector, such as those of GeneBio Systems' TA cloning vector (https://www.genebiosystems.com/50-ta-cloning-kits).
Performance data on this kit
Lyse n Amp(TM) Mouse Genotyping Kit was used to isolate DNA from mice and used to amplify 17 different gene fragments (from 200 bp to 800bp in length).
M: DL2,000 Plus DNA Marker
Literature citing the use of this kit:
1. Han W, He X, Zhang M, et al. (2015). Establishment of a porcine pancreatic stem cell line using T-RExTM system-inducible Wnt3a expression. Cell Prolif,48(3):301-310.
In addition, the same reagents were also used under a different product name to genotype mice:
2. Zhou L et al. Decorin promotes proliferation and migration of ORS keratinocytes and maintains hair anagen in mice. Exp Dermatol. 2018 Nov;27(11):1237-1244.
3. Li H et al., The effects of Nrf2 knockout on regulation of benzene-induced mouse hematotoxicity. Toxicol Appl Pharmacol. 2018 Nov 1;358:56-67.
4. Su D et al. One-step generation of mice carrying a conditional allele together with an HA-tag insertion for the delta opioid receptor.Sci Rep. 2017 Mar 16;7:44476.
5. Xiao J et al. Role of major and brain-specific Sgce isoforms in the pathogenesis of myoclonus-dystonia syndrome. Neurobiol Dis. 2017 Feb;98:52-65.
6. Xiao J et al. Motor phenotypes and molecular networks associated with germline deficiency of Ciz1. Exp Neurol. 2016 Sep;283(Pt A):110-20.
