Size: 50 rxn (50 ul/rxn)
The Vazyme HiScript II One Step RT-PCR SuperMix (Dye Plus) is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR SuperMix and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This SuperMix contains HiScript II Reverse Transcriptase, RNase inhibitor, and Champagne Taq plus hot-start DNA Polymerase, which enables high-sensitive total RNA detection (as little as 1 pg) and long-fragment amplification (as long as 10 kb). The 2× One Step Mix (Dye Plus) contains an opimized buffer, dNTPs, and Loading Dye.
|
components |
P612-01 50 rxn (50 ul/rxn) |
|
RNase free ddH2O |
1 ml×2 |
|
2×One Step Mixa |
625 μl×2 |
|
One Step Enzyme Mixb |
125 μl |
a. Contains dNTPs and Loading Dye.
b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.
Store at -20 C.