Size: 125 rxn (20 uL/reaction)
Validation Results:
A. Taking total RNA of Hela cells as template, the GAPDH fragment of 555 bp was amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The detection sensitivity can reach 0.1 pg.
B. Taking PPRSV RNA as template, a fragment of 660 bp was amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The detection sensitivity can reach 0.2 TCLD50.
C. Taking 1 μg of total RNA from Hela cells as template, fragments with different lengths were amplified using HiScript II One Step RT-PCR Kit (Vazyme, #P611). The UTRN fragments as long as 12.1 kb were successfully amplified.
The Vazyme HiScript II One Step RT-PCR Kit is specially designed for RNA detection (such as RNA virus). With the HiScript II One Step RT-PCR Kit and gene-specific primers (GSP), both reverse transcription and PCR amplification are performed in the same tube, with no additional pipetting procedures, which improves detection through-put and minimizes potential contamination. This kit contains HiScript II Reverse Transcriptase, Champagne Taq plus hot-start DNA Polymerase, and an optimized buffer, which enables high-sensitive total RNA detection and long-fragment amplification (as long as 10 kb).
|
Components |
P611-01 50 rxn (50 ul/rxn) |
|
RNase free ddH2O |
1 ml×2 |
|
2 × One Step Mixa |
1 ml×2 |
|
One Step Enzyme Mixb |
125 μl |
|
10 × Loading buffer |
1.25 ml |
a. Contains dNTPs.
b. Contains RNase inhibitor, HiScript II Reverse Transcriptase, and Champagne Taq plus Polymerase.
Storage: All components should be stored at -20°C.
