Introduction
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction or alcohol precipitation and involves minimal handling.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from100 mg simple plant |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount |
Fresh / frozen plant samples: ≤100m gDried plant / seed samples: ≤20mg |
| Elution volume | ≥30μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principle
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Advantages
- Safe - require no phenol or chloroform extraction
- Fast - several samples can be extracted in 30 minutes by silica technology
- High purity - high quality DNA, completely remove inhibitors
- High yield - silica technology can achieve the highest yield
Kit Contents
| Contents | D316402 | D316403 |
| Purification Times | 50 Preps | 250 Preps |
| RNase A |
10 mg |
50 mg |
| Protease Dissolve Buffer |
1.8 ml |
5 ml |
| Buffer SPL | 30 ml | 150 ml |
| Buffer PS | 10 ml | 50 ml |
| Buffer GW1 |
22 ml |
110 ml |
| Buffer GW2* | 12 ml | 2 x 50 ml |
| Buffer AE |
15 ml |
60 ml |
| HiPure gDNA Mini Columns |
50 | 2 x 125 |
| 2 ml Collection Tubes |
50 | 2 x 125 |
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
