Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis kit (AT311) to enable simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.
The users can optimize the reverse transcription primer, choosing between polydT and random 9-mers, as well as the primer concentration. In addition, the concentration of the transcriptase can also be optimized.
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.
Applications
Multiple copy and low copy gene detection
Storage
at -20 ℃ for two years
| Component | AT311-02 (50 rxns) | AT311-03 (100 rxns) | AT311-04 (500 rxns) |
| TransScript® RT/RI Enzyme Mix | 50 μl | 100 μl | 5×100 μl |
| gDNA Remover | 50 μl | 100 μl | 5×100 μl |
| 2×TS Reaction Mix | 500 μl | 1 ml | 5×1 ml |
| Random Primer(N9) (0.1 μg/μl) | 50 μl | 100 μl | 5×100 μl |
| Anchored Oligo(dT)18 Primer (0.5 μg/μl) | 50 μl | 100 μl | 5×100 μl |
| RNase-free Water | 500 μl | 1 ml | 5 ml |
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