This product delivers high efficiency of first strand cDNA synthesis. The 5× gDNA wiper Mix in the kit can quickly remove genomic DNA contamination at 42℃ for 2 min, which makes the results more reliable and simplifies qPCR primer design process without the need to design primers across introns. The kit contains two separated primers: the reverse transcription primers Oligo (dT) 20 VN and Random hexamers, allowing users to choose primers flexibly for subsequent experiments as needed. The kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize a highly uniform cDNA for qPCR quantification.
Components |
R312-01 50rxn (20μl/rxn) |
R312-02 100rxn (20μl/rxn) |
RNasefreeddH2O |
1 ml |
1 ml |
5× gDNAwiperMix |
100 μl |
200 μl |
10x RTMix |
100 μl |
200 μl |
GB-Script™ III EnzymeMix |
100 μl |
200 μl |
Oligo (dT)20VN |
50 μl |
100 μl |
RandomHexamers |
50 μl |
100 μl |