Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Cancer
Uniprot NO.:Q15465
Uniprot Entry Name:SHH_HUMAN
Gene Names:SHH
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:25-197aa
Sequence:IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:19.8 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 ?g/ml, corresponding to a specific activity of ? 1.0 × 103 IU/mg.
Purity:>98% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:SHH,
Relevance:Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity). {ECO:0000250}.
PubMed ID:7590746; 12853948; 12690205; 9593755; 7720571; 12837695; 16335952; 18417549; 19847792; 24456159; 24965254; 10753901; 19561609; 20504762; 8896572; 9302262; 10441331; 10556296; 11471164; 11479728; 12503095; 15103725; 15107988; 15221788; 15942952; 15942953; 16282375; 17001669; 19603532
Function:Sonic hedgehog protein
Involvement in disease:Microphthalmia, isolated, with coloboma, 5 (MCOPCB5); Holoprosencephaly 3 (HPE3); Solitary median maxillary central incisor (SMMCI); Triphalangeal thumb-polysyndactyly syndrome (TPTPS); Preaxial polydactyly 2 (PPD2); Hypoplasia or aplasia of tibia with polydactyly (THYP); Laurin-Sandrow syndrome (LSS)
Subcellular Location:Sonic hedgehog protein N-product: Cell membrane, Lipid-anchor
Protein Families:Hedgehog family
Tissue Specificity:
Paythway:Hedgehogsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:10848
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=164537
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:6469
STRING Database Link:https://string-db.org/network/9606.ENSP00000297261
OMIM Database Link:https://www.omim.org/entry/135750135750135750