Recombinant Human Protein disulfide-isomerase protein(P4HB) (Active)

Recombinant Human Protein disulfide-isomerase protein(P4HB) (Active)

CSB-AP000091HU
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Size:100ug. Other sizes are also available. Please contact us.

Research Areas:Signal Transduction

Uniprot NO.:P07237

Uniprot Entry Name:PDIA1_HUMAN

Gene Names:P4HB,ERBA2L,PDI,PDIA1,PO4DB

Species:Homo sapiens (Human)

Source:E.Coli

Expression Region:19-508aa

Sequence:MRGSGSHHHHHH+ APEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL

Protein Description:Partial

Tag Info:N-terminal 6xHis-tagged

Mol. Weight:56.6 kDa

Biological_Activity:Thiol Protein Reductase Activity is 0.001 ?650nm/ min-2, determined by measuring the turbidity increase at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time.

Purity:>95% as determined by SDS-PAGE and HPLC.

Endotoxin:Less than 1.0 EU/µg as determined by LAL method.

Form:Lyophilized powder

Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.0

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Alternative Name/ Alias:PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55,

Relevance:This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:12485997}.

PubMed ID:3034602; 3611107; 2846539; 14702039; 15489334; 1597478; 9150948; 12665801; 2079031; 1286669; 9399589; 3342239; 1699755; 10636893; 11181151; 12485997; 12095988; 12218051; 12218052; 12643545; 15158710; 14592831; 15644496; 17081065; 21269460; 24275569; 25683117; 8580850; 8672469; 10383197;

Function:This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration

Involvement in disease:Cole-Carpenter syndrome 1 (CLCRP1)

Subcellular Location:Endoplasmic reticulum, Endoplasmic reticulum lumen, Melanosome, Cell membrane, Peripheral membrane protein

Protein Families:Protein disulfide isomerase family

Tissue Specificity:

Paythway:Proteinprocessinginendoplasmicreticulum

HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:8548

UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=464336

KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:5034

STRING Database Link:https://string-db.org/network/9606.ENSP00000327801

OMIM Database Link:https://www.omim.org/entry/112240112240112240

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