Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:P21246
Uniprot Entry Name:PTN_HUMAN
Gene Names:PTN,HBNF1,NEGF1
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:33-168aa
Sequence:GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:15.3 kDa
Biological_Activity:Fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been precoated with 100 µl/well of a solution of 510 µg/ml rHuPTN.
Purity:>96% as determined by SDSPAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 m filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:PTN, HBBM, Heparin-binding growth factor 8, HBGF-8, Heparin-binding growth-associated molecule
Relevance:Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. {ECO:0000269|PubMed:11278720, ECO:0000269|PubMed:1768439}.
PubMed ID:1701634; 2270483; 1768439; 1457401; 8484754; 9299545; 14702039; 12690205; 15489334; 2388713; 1733956; 8241100; 8484780; 11278720; 20873783
Function:Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells
Involvement in disease:
Subcellular Location:Secreted
Protein Families:Pleiotrophin family
Tissue Specificity:Osteoblast and brain.
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:9630
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=371249
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:5764
STRING Database Link:https://string-db.org/network/9606.ENSP00000341170
OMIM Database Link:https://www.omim.org/entry/162095162095162095