Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Neuroscience
Uniprot NO.:P55145
Uniprot Entry Name:MANF_HUMAN
Gene Names:MANF,ARMET,ARP
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:25-182aa
Sequence:LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:18.2 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 ?g/ml, corresponding to a specific activity of ? 50 IU/mg.
Purity:>95% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:Arginine-rich protein,
Relevance:Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. {ECO:0000250, ECO:0000269|PubMed:12794311, ECO:0000269|PubMed:18561914}.
PubMed ID:8649854; 15489334; 16641997; 12665801; 12794311; 15144186; 18561914; 21269460; 24275569; 19258449; 8971156; 9174057
Function:Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:ARMET family
Tissue Specificity:
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:15461
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=436446
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:7873
STRING Database Link:https://string-db.org/network/9606.ENSP00000432799
OMIM Database Link:https://www.omim.org/entry/601916601916601916