Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:Q16674
Uniprot Entry Name:MIA_HUMAN
Gene Names:MIA
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:25-131aa
Sequence:GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:12.1 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 ?g/ml, corresponding to a specific activity of ? 200 IU/mg.
Purity:>98% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:Melanoma inhibitory activity protein,
Relevance:Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
PubMed ID:7923218; 8550608; 12230496; 15489334; 15340161; 19807924; 22419821; 11331761
Function:Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:MIA/OTOR family
Tissue Specificity:All malignant melanoma cell lines tested and infrequently in glioma cell lines.
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:7076
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=646364
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:8190
STRING Database Link:https://string-db.org/network/9606.ENSP00000263369
OMIM Database Link:https://www.omim.org/entry/601340601340601340