Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Cancer
Uniprot NO.:P09382
Uniprot Entry Name:LEG1_HUMAN
Gene Names:LGALS1
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:2-135aa
Sequence:ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:14.6 kDa
Biological_Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/ml.
Purity:>95% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 ?m filtered PBS, pH 7.4, with 1 mM DTT
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:Gal-1, HLBP14, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin
Relevance:May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. {ECO:0000269|PubMed:14617626, ECO:0000269|PubMed:18796645, ECO:0000269|PubMed:19497882}.
PubMed ID:2719964; 2910856; 2719646; 18824694; 1988031; 12761501; 15461802; 14702039; 10591208; 15489334; 3065332; 12665801; 3611046; 1386213; 2383549; 7501023; 10369126; 10764829; 11146440; 14617626; 19497882; 19608861; 21269460; 22905912; 22223895; 22814378; 23131994; 24275569; 26091039; 15476813; 18796645; 19128029
Function:Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Involvement in disease:
Subcellular Location:Secreted, extracellular space, extracellular matrix
Protein Families:
Tissue Specificity:Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:6561
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=445351
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3956
STRING Database Link:https://string-db.org/network/9606.ENSP00000215909
OMIM Database Link:https://www.omim.org/entry/150570150570150570