Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Cancer
Uniprot NO.:P29965
Uniprot Entry Name:CD40L_HUMAN
Gene Names:CD40LG,CD40L,TNFSF5,TRAP
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:113-261aa (C194W)
Sequence:MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:16.3 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.
Purity:>95% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.0
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:CD40-L, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD antigen CD154
Relevance:Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. {ECO:0000269|PubMed:15193700}.; Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. {ECO:0000269|PubMed:15193700}.
PubMed ID:1280226; 1385114; 7678782; 1281209; 7678552; 7999797; 15489334; 8626375; 11676606; 15193700; 8589998; 9605317; 7679206; 8094231; 7679801; 7717401; 7532185; 8889581; 8550833; 9150729; 9746782
Function:Cytokine that binds to CD40/TNFRSF5
Involvement in disease:Immunodeficiency with hyper-IgM, type 1 (HIGM1)
Subcellular Location:Cell membrane, Single-pass type II membrane protein, Cell surface, SUBCELLULAR LOCATION: CD40 ligand, soluble form: Secreted
Protein Families:Tumor necrosis factor family
Tissue Specificity:Specifically expressed on activated CD4+ T-lymphocytes.
Paythway:NF-kappaBsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:11935
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=592244
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:959
STRING Database Link:https://string-db.org/network/9606.ENSP00000359663
OMIM Database Link:https://www.omim.org/entry/300386300386300386