Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:Q16627
Uniprot Entry Name:CCL14_HUMAN
Gene Names:CCL14,NCC2,SCYA14
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:22-93aa
Sequence:TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:8.4 kDa
Biological_Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml.
Purity:>96% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:Chemokine CC-1/CC-3, HCC-11-74 NCC-2, Small-inducible cytokine A14
Relevance:Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. {ECO:0000269|PubMed:11085751}.
PubMed ID:8551235; 9600961; 10213461; 15489334; 10978165; 11085751; 17691823
Function:Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:Intercrine beta (chemokine CC) family
Tissue Specificity:Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma.
Paythway:Chemokinesignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:10612
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=272493
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:6358
STRING Database Link:
OMIM Database Link:https://www.omim.org/entry/601392601392601392