Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Microbiology
Uniprot NO.:P60022
Uniprot Entry Name:DEFB1_HUMAN
Gene Names:DEFB1,BD1,HBD1
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:22-68aa
Sequence:GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:5.1 kDa
Biological_Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.
Purity:>98% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:BD-1, Defensin, beta 1
Relevance:Has bactericidal activity. {ECO:0000250}.
PubMed ID:9268634; 9070620; 15489334; 7628632; 12010514; 11486002; 11714914
Function:Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility
Involvement in disease:
Subcellular Location:Secreted, Membrane
Protein Families:Beta-defensin family
Tissue Specificity:Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:2766
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=32949
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:1672
STRING Database Link:https://string-db.org/network/9606.ENSP00000297439
OMIM Database Link:https://www.omim.org/entry/602056602056602056