Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:ERN2,ERN1
GeneID:10595,2081
Uniprot:O75460/Q76MJ5
Specificity:Phospho-IRE1 (Ser724) Antibody detects endogenous levels of IRE1 only when phosphorylated at phospho S724.
Immunogen:A synthesized peptide derived from human IRE1 around the phosphorylation site of phospho S724.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Mouse,Rat
Prediction:Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
Application:WB,IHC,IF/ICC,ELISA(peptide)
Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Conjugate:Unconjugated
RRID:AB_2843590
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:AF7150-BP
Des# Blocking Peptide:Phospho-IRE1 (Ser724) Blocking peptide
Blocking Peptide Price:$549 CAD