The GB-Ruler™ 1kb Ladder Plus is ideal for determining the size of double-stranded DNA from 100 to 10,000 base pairs. The ladder consists of 15 linear double-stranded fragments. The 500bp and 3,000bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500bp and 3,000bp are 40ng. The 500bp and 3,000bp fragment are 100ng.
This ladder is pre-mixed with loading dye and is ready to use. Ready to load to the gel. Gel loading dye already added.
Recommended Loading
2-5 µl/Lane
Concentration
Typical Bands 100 ng/5µl
Other Bands 40 ng/5µl
Advised Electrophoresis Condition
8 cm, 1% Agarose Gel, 1×TAE, 7V/cm, 45min
Contents（bp）
100、200 、300、400、500、700、1,000、1,500、2,000、3,000、4,000、5,000、6,000、8,000、10,000
Mix Concentration
144 ng/µl
Storage
Stable for 3 months at RT, for long-time storage, please store at -20℃.
