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Description
GB-Amp™ Taq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has a 5' → 3' DNA polymerase and a 5' → 3' exonuclease activity but lacks a 3' → 5' exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase is heat-stable.
Contents
Taq DNA Polymerase by default is packaged in 5 U/μl.
10×PCR Buffer with Mg++,
Applications
Standard PCR
DNA labeling
DNA sequencing
Numerous applications for which a high-quality, thermostable DNA polymerase is required
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM TrisCl ( pH8.0), 100mM KCl, 3.2mM MgCl2 1mM DTT，0.1% Triton X-100 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol
Note
-Recombinant Taq DNA Polymerase is the enzyme of choice for most PCR applications.
-The half-life of enzyme is >40 minutes at 95°C.
-The error rate of Taq DNA Polymerase in PCR is 2.2x10-5 errors per nt per cycle;
the accuracy (an inverse of the error rate) an average number of correct nucleotides incorporated before making an error, is 4.5x104.
- Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as substrates for the DNA synthesis.Compatible with TA cloning – generates PCR products with 3’-dA overhangs.
-Suitable for Taqman probe real time PCR
-Recommendations with Template DNA in a 50μl reaction volume
-recommended quantities of template per react
Store all components at –20°C
