TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )-AT341
- Specification：
- 50 rxns×20 µl system
- 100 rxns×20 µl system
- 500 rxns×20 µl system
The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR (and regular end-point PCR)
• Simultaneous genomic DNA removal and cDNA synthesis.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.
Applications
Multiple copy and low copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AT341-01 (50 rxns ) | AT341-02 (100 rxns ) | AT341-03 (500 rxns ) |
| 5×TransScript® All-in-One SuperMix for qPCR | 200 μl | 400 μl | 5×400 μl |
| 5×TransScript® All-in-One No-RT Control SuperMix for qPCR | 20 μl | 40 μl | 200 μl |
| gDNA Remover | 50 μl | 100 μl | 5×100 μl |
| RNase-free Water | 1 ml | 2×1 ml | 2×5 ml |
Han X , Wang R , Zhou Y , et al. Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell, 2018, 172(5):1091-1107.e17.
Tian C , Zhang X , He J , et al. An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation[J]. Molecular Systems Biology, 2014, 10(10):755-755.
Wang J , Zhang Y , Hou J , et al. Ube2s regulates Sox2 stability and mouse ES cell maintenance[J]. Cell death and differentiation, 2015, 23(2).
Zhang L , Zhao X , Zhang G , et al. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0[J]. Scientific Reports, 2016, 6:20761.
Li P , Shi M L , Shen W L , et al. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2017, 1860(8):885-893.