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Description |
TransNGSTM Library Quantification qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a novel TransStart® TipTaq DNA Polymerase, unique hot start reagents (DNA binding proteins combined with unique chemical), EvaGreen, dNTPs, PCR Enhancer, PCR stabilizer and optimized reaction buffer suitable to NGS library quantification.TransNGSTM Library Quantification qPCR SuperMix enables accurate library amplification with various GC contents. It’s provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O. |
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Hightlights |
• Accurate library amplification with various GC contents Much stronger and more reliable signal with EvaGreen fluorescent dye. • Passive Reference Dyes for different qPCR instruments. |
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Storage |
at -20°C in dark for one year |
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Kit Content |
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Component |
KQ201-01 |
KQ201-02 |
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TransNGSTM Library Quantification qPCR SuperMix (2×) |
1 ml |
5×1 ml |
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Library Quantification Primer Mix (20×) |
100 μl |
500 μl |
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Passive Reference Dye (50×) |
40 μl |
200 μl |
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ddH2O |
1 ml |
5 ml |