Annexin V is one of the most sensitive indicators for early apoptosis detection. In normal cells, the phosphatidylserine (PS) is located on the inside of the phospholipid bilayer of the cell membrane. At the early stages of apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. The Annexin V, a Ca2+ dependent phospholipid-binding protein, can bind specifically to the exposed PS. Therefore, the nature of Annexin V binding to the PS exposed on the outside of cells can be used to detect the early apoptosis of the cells. Annexin V marked with R-Phycoerythrin (R-PE) is used as a probe to detect the occurrence of apoptosis using flow cytometry.
7-AAD is a nucleic acid binding dye, which cannot pass through the normal cells with complete cell membrane and early apoptotic cells, but can stain the nucleus through the cell membrane of apoptotic cells in the middle and late period and necrotic cells, and can be excited by 488 nm with a maximum emission wavelength of 647 nm. By maching Annexin V with 7-AAD, cells in different stages of apoptosis can be distinguished. This kit is easy to operate, highly sensitive and specific, and is suitable for the detection of early apoptosis of the cells.
Storage
at 2-8℃ for one year.
Shipping
Ice bag (4℃).
| Component |
FA121-01 (25 rxns) |
FA121-02 (50 rxns) |
| Annexin V-PE |
125 μl |
250 μl |
| 7-AAD |
125 μl | 250 μl |
|
1×Annexin V Binding Buffer |
12.5 ml | 2×12.5 ml |