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Supplied in 2 x 50μl reactions per vial; 100μl per vial.
20 reaction size is delivered in 10 vials; 40 reaction size is delivered in 20 vials.
Description
Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA).
Genotype
F-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (rk-, mk+) supE44λ- thi-1 gyrA96relA1 phoA
Features
- High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
- Reduced recombination of cloned DNA.
- Blue/white selection.
Storage
Competent Cell at -70°C for six months
Notes
Higher efficiency transformation can be achieved by transforming cells immediately following thawing.
Avoid repeated thawing.
Gentle handling is required for the entire procedure.
References:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Fungicides induced triazole-resistance in Aspergillus fumigatus associated with mutations of TR46/Y121F/T289A and its appearance in agricultural fields | Journal of Hazardous Materials | 6.065 | Jingbei Ren,et al. | 2017Mar | The original link |
| Automatic Enumeration of Gold Nanomaterials at the Single-Particle Level | Anal Chem | 5.886 | Xiao Xu, et al. | 2015 Feb | The original link |
| Utilization of biodiesel by-product as substrate for high-production of ??-farnesene via relatively balanced mevalonate pathway in Escherichia coli | Bioresource Technology | 5.65 | Shengping You et al | 2017 Jun | The original link |
| The antitumor activity and preliminary modeling on the potential mechanism of action of human peroxiredoxin-5 | Oncotarget | 5.1657 | Juanjuan Liu,et al. | 2017Mar | The original link |
| Monitoring and evaluation of antibiotic resistance genes in four municipal wastewater treatment plants in Harbin, Northeast China | Environ Pollut | 5.0989 | Wen Q, et al. | 2016 Jan | The original link |