Additional $40 shipping charge required for dry ice shipping
Supplied in 2 x 50μl reactions per vial; 100μl per vial.
20 reaction size is delivered in 10 vials; 40 reaction size is delivered in 20 vials.
Description
Trans1-T1 Phage Resistant Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 109 cfu/μg DNA (tested by pUC19 plasmid DNA).
Genotype
F-φ80(lacZ)ΔM15ΔlacX74hsdR(rk-, mk+)ΔrecA1398endA1tonA
Features
- High transformation efficiency: >109 cfu/μg (pUC19 DNA).
- Fast-growing, colonies are visible in 8~9 hours.
- Resistance to T1 and T5 phage.
- Blue/white selection.
Storage
Competent Cell at -70°C for six months
Notes
Higher efficiency transformation can be achieved by transforming cells immediately following thawing.
Avoid repeated thawing.
Gentle handling is required for the entire procedure.
References:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Removal of antibiotic resistance genes from wastewater treatment plant effluent by coagulation | water research | 6.9419 | Na Li,et al. | 2017Mar | The original link |
| Production and characterization of poly(3-hydroxypropionate-co-4-hydroxybutyrate) with fully controllable structures by recombinant Escherichia coli containing an engineered pathway | Metabolic Engineering | 6.859 | Meng DC, et al. MOE Key Lab of Bioinformatics, Dep | 2012 Jul | The original link |
| Absolute Quantification of H5-subtype Avian Influenza Viruses Using Droplet Digital Loop-mediated Isothermal Amplification | analytical chemistry | 6.32 | Yi Hu,et al. | 2016Nov | The original link |
| Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years | journal of hazardous materials | 6.065 | Shuang Peng,et al. | 2017Qct | The original link |
| Effect of fumigation with chloropicrin on soil bacterial communities and genes encoding key enzymes involved in nitrogen cycling | Environmental Pollution | 5.0989 | Jun Li,et al. | 2017Aug | The original link |