Introduction
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil samples. Up to 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatilityof spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation DNA from 200-500mg soil sample |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Soil |
| Sample amount |
200-500mg |
| Elution volume | ≥30μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principle
Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Advantages
- Fast - several samples can be extracted in 40 minutes (after digestion)
- High purity - purified DNA can be directly used in various downstream applications
- Good repeatability - silica technology can obtain ideal results every time
- High recovery - DNA can be recovered at the level of PG
Kit Contents
| Contents | D314202 | D314203 |
| Number of Preps |
50 Preps | 250 Preps |
| Hipure DNA Mini Columns II | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| 2ml Bead Tubes |
50 |
250 |
| Buffer SQL |
60 ml |
250 ml |
| Buffer SDS | 5 ml | 20 ml |
| Buffer PS |
10 ml |
50 ml |
| Absorber Solution |
10 ml |
50 ml |
| Buffer GDP | 80 ml | 2 x 200 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| Buffer AE | 15 ml | 30 ml |
Storage and Stability
Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
