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Updated Date: Stock Protein updated on 20171018
Research areas: Others
Target / Protein: Mcpt2
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Rattus norvegicus (Rat)
Delivery time: 3-7 business days
Uniprot ID: P00770
AA Sequence: IIGGVESIPHSRPYMAHLDIVTEKGLRVICGGFLISRQFVLTAAHCKGREITVILGAHDVRKRESTQQKIKVEKQIIHESYNSVPNLHDIMLLKLEKKVELTPAVNVVPLPSPSDFIHPGAMCWAAGWGKTGVRDPTSYTLREVELRIMDEKACVDYRYYEYKFQVCVGSPTTLRAAFMGDSGGPLLCAGVAHGIVSYGHPDAKPPAIFTRVSTYVPWINAVINTSS
Tag info: N-terminal 6xHis-tagged and C-terminal Myc-tagged
Expression Region: 21-247aa
Protein length: Full Length of Mature Protein
MW: 30.5 kDa
Alternative Name(s): Group-specific protease Mast cell protease II
Relevance: This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.
Reference: "Covalent structure of a group-specific protease from rat small intestine. Appendix: crystallographic data for a group specific protease from rat intestine." Woodbury R.G., Katunuma N., Kobayashi K., Titani K., Neurath H. Biochemistry 17:811-819(1978)
Purity: Greater than 85% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.