Recombinant Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial(Pdk1)

CSB-BP807321MO

>Several Other Sizes Are Also Available. Please Inquire. Default Size: 20ug

Updated Date: Stock Protein updated on 20170725

Research areas: Signal Transduction

Target / Protein: Pdk1

Biologically active: Not Tested

Expression system: Baculovirus

Species of origin: Mus musculus(Mouse)

Delivery time: 3-7 business days

Uniprot ID: Q8BFP9

AA Sequence: ASASASGSGPASELGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESVERLPVYNKAAWKHYKANHEADDWCVPSREPKDMTTFRSS

Tag info: N-terminal 10xHis-tagged

Expression Region: 27-434aa

Protein length: Full Length of Mature Protein

MW: 48.7 kDa

Alternative Name(s): Pyruvate dehydrogenase kinase isoform 1

Relevance: Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress

Reference: "HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumption." Papandreou I., Cairns R.A., Fontana L., Lim A.L., Denko N.C. Cell Metab. 3:187-197(2006)

Purity: Greater than 85% as determined by SDS-PAGE.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.