Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:O35903
Uniprot Entry Name:CCL25_MOUSE
Gene Names:Ccl25,Scya25,Teck
Species:Mus musculus (Mouse)
Source:E.Coli
Expression Region:24-144aa
Sequence:QGAFEDCCLG YQHRIKWNVL RHARNYHQQE VSGSCNLRAV RFYFRQKVVC GNPEDMNVKR AIRILTARKR LVHWKSASDS QTERKKSNHM KSKVENPNST SVRSATLGHP RMVMMPRKTN N
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:14.1 kDa
Biological_Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-50 ng/ml.
Purity:>95% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:Chemokine TECK, Thymus-expressed chemokine
Relevance:Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
PubMed ID:9285413; 10602049; 16517733; 16141072; 19468303; 15489334
Function:Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:Intercrine beta (chemokine CC) family
Tissue Specificity:Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Paythway:
HGNC Database Link:
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Mm&CID=7275
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?mmu:20300
STRING Database Link:https://string-db.org/network/10090.ENSMUSP00000024004
OMIM Database Link: