Size: 200ug. Other sizes are also available. Please Inquire.
In Stock: No
Lead time: 10-20 working days
Research Topic: Others
Uniprot ID: P16980
Gene Names: NP
Organism: Influenza A virus (strain A/Equine/Prague/1/1956 H7N7)
AA Sequence: MASQGTKRPYEQMETGGERQNATEIRASVGKMVGGIGRFYIQMCTELKLNDYEGRLIQNSITIEKMVLSAFDERRNKYLEEHPNTGKDPKKTGGPIYRKREGKWIRELILYDKEEIRRIWRQANNGEDATAGLTHLMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLIVASGYDFEREGYSLVGVDPFKLLQNSQIFSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTKVIPRGQLSTRGIQIASNENMETIDSNTLELRSRYWAIRTKSGGNTSQQKASAGQISVQPTFSVQRNLPFERTTIMAAFTGNNEGRTSDMRTEIIRMMENAKPDDVSFQGRGVFELSDEKATNPIVPSFDMSKEGSYFFGDNAEEFDN
Expression Region: 1-498aa
Sequence Info: Full Length
Source: E.coli
Tag Info: N-terminal 6xHis-SUMO-tagged
MW: 72.2 kDa
Alternative Name(s): Nucleocapsid protein ;Protein N
Relevance: Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as tplate for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP ses to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus .
Reference: Large-scale sequence analysis of avian influenza isolates.Obenauer J.C., Denson J., Mehta P.K., Su X., Mukatira S., Finkelstein D.B., Xu X., Wang J., Ma J., Fan Y., Rakestraw K.M., Webster R.G., Hoffmann E., Krauss S., Zheng J., Zhang Z., Naeve C.W.Science 311:1576-1580(2006)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage Buffer: Tris-based buffer，50% glycerol
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.