Recombinant Human SARS coronavirus Spike glycoprotein(S) ,partial (Active)

Recombinant Human SARS coronavirus Spike glycoprotein(S) ,partial (Active)

CSB-MP348663HQE
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Size:100ug. Other sizes are also available. Please contact us.

Research Areas:Cancer

Uniprot NO.:P59594

Uniprot Entry Name:

Gene Names:S

Species:Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)

Source:Mammalian cell

Expression Region:306-527aa

Sequence:RVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNF

Protein Description:Extracellular domain

Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged

Mol. Weight:30 kDa

Biological_Activity:?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 ?g/ml can bind Paguma larvata ACE2 (CSB-MP684964PAL), the EC50 is 5.056-7.559 ng/ml.?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 ?g/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 7.941-10.49 ng/ml.

Purity:Greater than 95% as determined by SDS-PAGE.

Endotoxin:Less than 1.0 EU/ug as determined by LAL method.

Form:Lyophilized powder

Buffer:Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

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