Size:100ug. Other sizes are also available. For further information, please contact us.
Research Areas:Cardiovascular
Uniprot ID:P34096
Gene Names:RNASE4
Organism:Homo sapiens (Human)
AA Sequence:QDGMYQRFLRQHVHPEETGGSDRYCNLMMQRRKMTLYHCKRFNTFIHEDIWNIRSICSTTNIQCKNGKMNCHEGVVKVTDCRDTGSSRAPNCRYRAIASTRRVVIACEGNPQVPVHFDG
Expression Region:29-147aa
Sequence Info:Full Length of Mature Protein
Source:Baculovirus
Tag Info:N-terminal MBP-tagged and C-terminal 6xHis-tagged
MW:57.8 kDa
Alternative Name(s):RNS4
Relevance:This RNase has marked specificity towards the 3' side of uridine nucleotides.
Reference:"Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues." Rosenberg H.F., Dyer K.D. Nucleic Acids Res. 23:4290-4295(1995)
Purity:Greater than 85% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:This RNase has marked specificity towards the 3' side of uridine nucleotides.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:Pancreatic ribonuclease family
Tissue Specificity:
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:10047
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=283749
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:6038
STRING Database Link:https://string-db.org/network/9606.ENSP00000381081
OMIM Database Link:https://www.omim.org/entry/601030601030601030
Lead Time Guidance:28-38 business days