Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial,Biotinylated (Active)

Recombinant Human Novel Coronavirus Spike glycoprotein(S),partial,Biotinylated (Active)

CSB-MP3324GMY1-B
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Size:100ug. Other sizes are also available. Please contact us.

Research Areas:Others

Uniprot NO.:P0DTC2

Uniprot Entry Name:

Gene Names:S

Species:Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Source:Mammalian cell

Expression Region:319-541aa

Sequence:RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Protein Description:Partial

Tag Info:C-terminal mFc-Avi-tagged

Mol. Weight:54.2 kDa

Biological_Activity:Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 ?g/ml can bind Biotinylated-SARS-CoV-2-S1-RBD (CSB-MP3324GMY1-B), the EC50 is 5.087-7.050 ng/ml.

Purity:Greater than 85% as determined by SDS-PAGE.

Endotoxin:Less than 1.0 EU/ug as determined by LAL method.

Form:Lyophilized powder

Buffer:Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

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