Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:P15018
Uniprot Entry Name:LIF_HUMAN
Gene Names:LIF,HILDA
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:23-202aa
Sequence:SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:19.7 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of ? 1.0 × 107 IU/mg.
Purity:>98% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 ?m filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:LIF, D factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin
Relevance:LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
PubMed ID:3143918; 2475312; 15146197; 1692837; 14702039; 10591208; 15489334; 3128791; 2730639; 8489250; 14527405; 17652170
Function:LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:LIF/OSM family
Tissue Specificity:
Paythway:Jak-STATsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:6596
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=2250
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3976
STRING Database Link:https://string-db.org/network/9606.ENSP00000249075
OMIM Database Link:https://www.omim.org/entry/159540159540159540