Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:P10145
Uniprot Entry Name:IL8_HUMAN
Gene Names:CXCL8,IL8
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:23-99aa
Sequence:AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:8.9 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/ml, corresponding to a specific activity of ? 5.0 ×105 IU/mg.
Purity:>96% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IL-8, Chemokine C-X-C motif, ligand 8, Emoctakin, Granulocyte chemotactic protein 1
Relevance:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. {ECO:0000269|PubMed:11978786, ECO:0000269|PubMed:2145175, ECO:0000269|PubMed:2212672}.
PubMed ID:2953813; 3260265; 2663993; 2664463; 2200751; 14702039; 15489334; 2523801; 2145175; 2648135; 2659722; 1755384; 2655583; 8344717; 3279957; 3480540; 3322281; 2212672; 2007144; 11023497; 11978786; 1639201; 14711052; 18710930; 19608678; 20829347; 2681204; 2184886; 8631339; 10368283; 2182630; 2005614; 1988949; 10707023
Function:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:Intercrine alpha (chemokine CxC) family
Tissue Specificity:
Paythway:Chemokinesignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:6025
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=624
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3576
STRING Database Link:https://string-db.org/network/9606.ENSP00000306512
OMIM Database Link:https://www.omim.org/entry/146930146930146930