Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Cancer
Uniprot NO.:P01344
Uniprot Entry Name:IGF2_HUMAN
Gene Names:IGF2,PP1446
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:25-91aa
Sequence:AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:7.5 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of ? 5.0 × 105 IU/mg.
Purity:>98% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IGF-II, T3M-11-derived growth factor,
Relevance:The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
PubMed ID:6382022; 6382021; 2450353; 3002851; 3476948; 3683205; 3881277; 7730145; 16531418; 20842449; 15498874; 14702039; 16554811; 15489334; 3167054; 3653397; 3652904; 658418; 2722836; 7633596; 11448941; 12586351; 15359740; 1569071; 16912056; 19066168; 19838169; 22171320; 23234360; 6189745; 7527339; 18046459
Function:The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF-II is influenced by placental lactogen. Also involved in tissue differentiation. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation (By similarity). In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver (Probable).
Involvement in disease:Silver-Russell syndrome (SRS); Growth restriction, severe, with distinctive facies (GRDF)
Subcellular Location:Secreted
Protein Families:Insulin family
Tissue Specificity:Expressed in heart, placenta, lung, liver, muscle, kidney, tongue, limb, eye and pancreas.
Paythway:MAPKsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5466
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=272259
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3481
STRING Database Link:https://string-db.org/network/9606.ENSP00000391826
OMIM Database Link:https://www.omim.org/entry/147470147470147470