Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Cancer
Uniprot NO.:P05019
Uniprot Entry Name:IGF1_HUMAN
Gene Names:IGF1,IBP1
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:52-118aa
Sequence:TLCGAELVDA LQFVCGDRGF YFNKPTGYGS SSRRAPQTGI VDECCFRSCD LRRLEMYCAP LKPAKSA
Protein Description:Partial
Tag Info:Tag-Free
Mol. Weight:7.4 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of ? 5.0 × 105 IU/mg.
Purity:>97% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IGF-I, MGF, Somatomedin-C
Relevance:The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. {ECO:0000269|PubMed:21076856, ECO:0000269|PubMed:24132240}.
PubMed ID:6358902; 3002851; 2935423; 2937782; 3455760; 2082190; 2018498; 1372070; 8495408; 16541075; 15489334; 6382022; 632300; 3242681; 21076856; 24132240; 6189745; 2036417; 1319992; 8857020; 10391209
Function:The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation
Involvement in disease:Insulin-like growth factor I deficiency (IGF1 deficiency)
Subcellular Location:Secreted
Protein Families:Insulin family
Tissue Specificity:
Paythway:HIF-1signalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5464
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=160562
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3479
STRING Database Link:https://string-db.org/network/9606.ENSP00000302665
OMIM Database Link:https://www.omim.org/entry/147440147440147440