Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Cancer
Uniprot NO.:P24593
Uniprot Entry Name:IBP5_HUMAN
Gene Names:IGFBP5,IBP5
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:21-272aa
Sequence:LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:28.6 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 ?g/ml, corresponding to a specific activity of ? 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
Purity:>96% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.0
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IBP-5, IGFBP-5
Relevance:IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
PubMed ID:1850258; 1709938; 7511611; 15489334; 1850257; 9883900; 22117064; 26091039; 9822601
Function:IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:
Tissue Specificity:Osteosarcoma, and at lower levels in liver, kidney and brain.
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5474
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=607212
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3488
STRING Database Link:https://string-db.org/network/9606.ENSP00000233813
OMIM Database Link:https://www.omim.org/entry/146734146734146734