Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Cancer
Uniprot NO.:P17936
Uniprot Entry Name:IBP3_HUMAN
Gene Names:IGFBP3,IBP3
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:28-291aa
Sequence:GASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:28.8 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of ? 5.0 × 103 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
Purity:>98% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IBP-3, IGFBP-3
Relevance:IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. {ECO:0000269|PubMed:20353938}.
PubMed ID:2464130; 1695633; 7508771; 12853948; 12690205; 15489334; 1697583; 1726837; 15340161; 14561895; 14760718; 16335952; 19159218; 19139490; 20353938; 26091039; 16959974
Function:IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:
Tissue Specificity:Expressed by most tissues. Present in plasma.
Paythway:p53signalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5472
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=450230
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3486
STRING Database Link:https://string-db.org/network/9606.ENSP00000370473
OMIM Database Link:https://www.omim.org/entry/146732146732146732