Size:20ug. Other sizes are also available. For further information, please contact us.
Research Areas:Neuroscience
Uniprot ID:Q05586
Gene Names:GRIN1
Organism:Homo sapiens (Human)
AA Sequence:RAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYSWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEAKELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGILGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTLSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQ
Expression Region:19-559aa
Sequence Info:Extracellular Domain
Source:E.coli
Tag Info:N-terminal 6xHis-tagged
MW:64.6 kDa
Alternative Name(s):Glutamate [NMDA] receptor subunit zeta-1 N-methyl-D-aspartate receptor subunit NR1 Short name: NMD-R1 NMDAR1
Relevance:Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+. Sensitivity to glutamate and channel kinetics depend on the subunit composition
Reference:"Inducible expression of neuronal glutamate receptor channels in the NT2 human cell line." Younkin D.P., Tang C.-M., Hardy M., Reddy U.R., Shi Q.-Y., Pleasure S.J., Lee V.M.-Y., Pleasure D. Proc. Natl. Acad. Sci. U.S.A. 90:2174-2178(1993)
Purity:Greater than 90% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+)
Involvement in disease:Neurodevelopmental disorder with or without hyperkinetic movements and seizures, autosomal dominant (NDHMSD)
Subcellular Location:Cell membrane, Multi-pass membrane protein, Cell junction, synapse, postsynaptic cell membrane, Cell junction, synapse, postsynaptic cell membrane, postsynaptic density
Protein Families:Glutamate-gated ion channel (TC 1.A.10.1) family, NR1/GRIN1 subfamily
Tissue Specificity:
Paythway:Calciumsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:4584
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=558334
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:2902
STRING Database Link:https://string-db.org/network/9606.ENSP00000360608
OMIM Database Link:https://www.omim.org/entry/138249138249138249
Lead Time Guidance:3-7 business days